pSYC-190
(Plasmid #178962)

• Purpose: Expresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cells
• Depositing Lab: Seok-Yong Choi
• Publication: Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 10.1186/s12883-017-0938-7.

Backbone

• Vector backbone: pCS4+3xFLAG-p2A
• Backbone size w/o insert (bp): 4326
• Total vector size (bp): 5622
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Glial fibrillary acidic protein
• Alt name: GFAP
• Species: H. sapiens (human)
• Insert Size (bp): 1296
• Mutation: R79C
• GenBank ID: NM_002055.5
• Entrez Gene: GFAP (a.k.a. ALXDRD)
• Promoter: CMV IE94 Promoter
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pSYC-190.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry