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Addgene

pYPQ-Cas9-Act3.0
(Plasmid #178954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ173
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
  • Species
    Synthetic
  • Insert Size (bp)
    11000

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ-Cas9-Act3.0 was a gift from Yiping Qi (Addgene plasmid # 178954 ; http://n2t.net/addgene:178954 ; RRID:Addgene_178954)

  • For your References section:

    Boosting plant genome editing with a versatile CRISPR-Combo system. Pan C, Li G, Malzahn AA, Cheng Y, Leyson B, Sretenovic S, Gurel F, Coleman GD, Qi Y. Nat Plants. 2022 May;8(5):513-525. doi: 10.1038/s41477-022-01151-9. Epub 2022 May 20. 10.1038/s41477-022-01151-9 PubMed 35596077
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