TLCV2-APOBEC1-YTHmut
(Plasmid #178950)

• Purpose: Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells
• Depositing Lab: Kate Meyer
• Publication: Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10. doi: 10.1016/j.molcel.2021.12.038. Epub 2022 Jan 25.

Backbone

• Vector backbone: TLCV2 (lentiCRISPR v2)
• Backbone manufacturer: Adam Karpf (Feng Zhang)
• Backbone size w/o insert (bp): 11775
• Total vector size (bp): 13823
• Vector type: Mammalian Expression, Lentiviral, Synthetic Biology ; Inducible
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: APOBEC1-YTHmut-T2A-GFP
• Species: H. sapiens (human), R. norvegicus (rat)
• Insert Size (bp): 2046
• Promoter: tight TRE
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: LNCX

Resource Information

• Supplemental Documents:
  • TLCV2_BE1_YTHmut.gb
• A portion of this plasmid was derived from a plasmid made by: APOBEC1-YTH is cloned from Addgene 131637, made by Kate Meyer
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry