pTN14
(Plasmid
#178945)
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PurposeExpresses AtTIR1(F79G) in C elegans
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTN14 was a gift from Masato Kanemaki (Addgene plasmid # 178945 ; http://n2t.net/addgene:178945 ; RRID:Addgene_178945) -
For your References section:
The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT. Genetics. 2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218. 10.1093/genetics/iyab218 PubMed 34865044