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Addgene

pAAV-mGrn-myc
(Plasmid #178944)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178944 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CIGW
  • Backbone size w/o insert (bp) 6585
  • Total vector size (bp) 8355
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grn
  • Alt name
    Progranulin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1770
  • Entrez Gene
    Grn (a.k.a. GP88, PCDGF, PEPI, Pgrn, epithelin)
  • Promoter CBA
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TACGCCGGCGTTAATGCATATTCAGATCCTCTTCTGAG
  • 3′ sequencing primer ATAGCGGCCGCTTAATGCATATTCAGATCCTCTTCTGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-mGrn-myc was a gift from Erik Roberson (Addgene plasmid # 178944 ; http://n2t.net/addgene:178944 ; RRID:Addgene_178944)

  • For your References section:

    Restoring neuronal progranulin reverses deficits in a mouse model of frontotemporal dementia. Arrant AE, Filiano AJ, Unger DE, Young AH, Roberson ED. Brain. 2017 May 1;140(5):1447-1465. doi: 10.1093/brain/awx060. 10.1093/brain/awx060 PubMed 28379303
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