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Addgene

BASIC_SEVA_37.10
(Plasmid #178925)

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Item Catalog # Description Quantity Price (USD)
Plasmid 178925 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    BASIC_SEVA_37.10
  • Backbone size w/o insert (bp) 2585
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mScarlet counter-selection cassette
  • Species
    Synthetic
  • Insert Size (bp)
    1074

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ggcggcggatttgtcctac
  • 3′ sequencing primer ggtgagaatccaggggtcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BASIC_SEVA_37.10 was a gift from Paul Freemont (Addgene plasmid # 178925 ; http://n2t.net/addgene:178925 ; RRID:Addgene_178925)

  • For your References section:

    basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method. Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont P, Storch M. Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 10.1093/synbio/ysac023. eCollection 2022. 10.1093/synbio/ysac023 PubMed 36381610
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