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psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut
(Plasmid #178910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3560
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The plasmid was prepped in HST08 strain for the original study.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3'UTR mMov10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    383
  • Mutation
    CTG>AGT and TGC>CAT mir16+153 mutated
  • Entrez Gene
    Mov10 (a.k.a. C77703, Mov-10)
  • Promoter SV40 promoter

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GGTTCTTTTCCAACGCTAT
  • 3′ sequencing primer CGAGGTCCGAAGACTCATT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.11.09.467897 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut was a gift from Constance Ciaudo (Addgene plasmid # 178910 ; http://n2t.net/addgene:178910 ; RRID:Addgene_178910)

  • For your References section:

    Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts retrotransposition. Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C. EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458. 10.15252/embr.202154458 PubMed 35856394
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