Skip to main content
Addgene

pcDNA3-T11HA-hMOV10-CD
(Plasmid #178908)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178908 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The plasmid was prepped in HST08 strain for the original study.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cDNA Catalytic dead hMOV10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3012
  • Mutation
    K530A catalytic dead AAG>GCA
  • Entrez Gene
    MOV10 (a.k.a. fSAP113, gb110)
  • Promoter CMV protmoter
  • Tag / Fusion Protein
    • T11 beta strand of GFP-HA (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer Bgh Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.11.09.467897 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-T11HA-hMOV10-CD was a gift from Constance Ciaudo (Addgene plasmid # 178908 ; http://n2t.net/addgene:178908 ; RRID:Addgene_178908)

  • For your References section:

    Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts retrotransposition. Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C. EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458. 10.15252/embr.202154458 PubMed 35856394
Related items: