FHRE-Luc
(Plasmid #1789)

• Purpose: Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.
• Depositing Lab: Michael Greenberg
• Publication: Brunet et al Cell 1999 Mar 19;96(6):857-68.

Backbone

• Vector backbone: pGL3 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FHRE
• Alt name: FKHRL1
• Alt name: FOXO3a
• Alt name: forkhead responsive element
• Species: H. sapiens (human)
• Insert Size (bp): 96
• Mutation: 3 copies of FRE
• Entrez Gene: FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: RVprimer3

Resource Information

• Articles Citing this Plasmid:
  • 43 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map and sequence.