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Addgene

pHN767
(Plasmid #178884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178884 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 7269
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Peptide:N-glycosidase F
  • Alt name
    PNGase F
  • Species
    Flavobacterium meningosepticum
  • Insert Size (bp)
    549
  • Promoter T7
  • Tag / Fusion Protein
    • GST, His8 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTTTTATACATGGACCCAATGTGC
  • 3′ sequencing primer CTTTCAGCAAAAAACCCCTCAAGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHN767 was a gift from Kazuhiro Abe (Addgene plasmid # 178884 ; http://n2t.net/addgene:178884 ; RRID:Addgene_178884)
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