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pCAGGS-PB1-MontiRed-LOV2dark
(Plasmid #178874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone manufacturer
    Dr. Jun-ichi Miyazaki (Osaka University, Japan)
  • Backbone size w/o insert (bp) 4091
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PB1-MontiRed-LOV2dark
  • Species
    Synthetic
  • Insert Size (bp)
    1852
  • Mutation
    LOV2: C450A, L514K, G528A, L531E, N538E
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-PB1-MontiRed-LOV2dark was a gift from Shinya Tsukiji (Addgene plasmid # 178874 ; http://n2t.net/addgene:178874 ; RRID:Addgene_178874)

  • For your References section:

    Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. Yoshikawa M, Yoshii T, Ikuta M, Tsukiji S. J Am Chem Soc. 2021 May 5;143(17):6434-6446. doi: 10.1021/jacs.0c12375. Epub 2021 Apr 23. 10.1021/jacs.0c12375 PubMed 33890764
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