pCMV-FKBP-mCherry-MCS
(Plasmid
#178863)
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PurposeMammalian expression of a protein of interest fused to the C-terminus of FKBP-mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4713
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKBP-mCherry
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SpeciesSynthetic
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Insert Size (bp)354
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-FKBP-mCherry-MCS was a gift from Shinya Tsukiji (Addgene plasmid # 178863 ; http://n2t.net/addgene:178863 ; RRID:Addgene_178863) -
For your References section:
Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. Yoshikawa M, Yoshii T, Ikuta M, Tsukiji S. J Am Chem Soc. 2021 May 5;143(17):6434-6446. doi: 10.1021/jacs.0c12375. Epub 2021 Apr 23. 10.1021/jacs.0c12375 PubMed 33890764