pCMV-miRFP670-eDHFR(69K6)
(Plasmid
#178859)
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PurposeMammalian expression of miRFP670-eDHFR(69K6)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3925
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiRFP670-eDHFR(69K6)
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SpeciesSynthetic
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Insert Size (bp)1511
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-miRFP670-eDHFR(69K6) was a gift from Shinya Tsukiji (Addgene plasmid # 178859 ; http://n2t.net/addgene:178859 ; RRID:Addgene_178859) -
For your References section:
A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation. Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, Atsuta-Tsunoda K, Aoki K, Tsukiji S. Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13. 10.1016/j.chembiol.2022.06.005 PubMed 35835118