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pCAGGS-chGrb2/eDHFR(69K6)-iRFP713
(Plasmid #178854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone manufacturer
    Dr. Jun-ichi Miyazaki (Osaka University, Japan)
  • Backbone size w/o insert (bp) 4880
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1966
  • Mutation
    Grb2: Y160E
  • Entrez Gene
    GRB2 (a.k.a. ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-chGrb2/eDHFR(69K6)-iRFP713 was a gift from Shinya Tsukiji (Addgene plasmid # 178854 ; http://n2t.net/addgene:178854 ; RRID:Addgene_178854)

  • For your References section:

    A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation. Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, Atsuta-Tsunoda K, Aoki K, Tsukiji S. Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13. 10.1016/j.chembiol.2022.06.005 PubMed 35835118
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