pHN1002
(Plasmid #178831)

• Purpose: Bacterial expression for human Saposin A
• Depositing Lab: Kazuhiro Abe
• Publication: Bacterial expression for human Saposin A (unpublished)

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Total vector size (bp): 5944
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: artificial gene encoding human Saposin A
• Alt name: hSapA_60-140
• Species: H. sapiens (human)
• Insert Size (bp): 294
• Mutation: Codon-usage was optimized for bacterial expression. N terminal propeptide (59aa) was deleted.
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: T7
• Tag / Fusion Protein:
  • His8, 3C (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTAGAGGATCGAGATCTCGATCCC
• 3′ sequencing primer: CTTTCAGCAAAAAACCCCTCAAGAC

Resource Information

• Supplemental Documents:
  • pHN1002_pET15b_H8_3C_SapA .gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry