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Addgene

pHN872
(Plasmid #178830)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178830 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 6601
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    artificial gene encoding human Saposin A
  • Alt name
    hSapA_60-140
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    294
  • Mutation
    Codon-usage was optimized for bacterial expression. N terminal propeptide (59aa) was deleted.
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter T7
  • Tag / Fusion Protein
    • GST, His8, TEV (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAGAGGATCGAGATCTCGATCCC
  • 3′ sequencing primer CTTTCAGCAAAAAACCCCTCAAGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHN872 was a gift from Kazuhiro Abe (Addgene plasmid # 178830 ; http://n2t.net/addgene:178830 ; RRID:Addgene_178830)
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