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Addgene

pHN764
(Plasmid #178819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMal_c2
  • Backbone manufacturer
    NEB
  • Total vector size (bp) 7480
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic gene encoding endo-β-N-acetylglucosaminidase H
  • Alt name
    EndoH
  • Species
    Streptomyces sp.
  • Insert Size (bp)
    816
  • Promoter tac
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • HIs8 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATGAAGCCCTGAAAGACGCGC
  • 3′ sequencing primer CGCCAGGGTTTTCCCAGTCACGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHN764 was a gift from Kazuhiro Abe (Addgene plasmid # 178819 ; http://n2t.net/addgene:178819 ; RRID:Addgene_178819)
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