FLAG-mBAF45b IRES GFP
(Plasmid
#17881)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBeta-actin/CMV-IRES-EGFP
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBAF45b
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Alt nameNeud4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1166
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Entrez GeneDpf1 (a.k.a. Neud4)
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- IRES EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (destroyed during cloning)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-mBAF45b IRES GFP was a gift from Jerry Crabtree (Addgene plasmid # 17881 ; http://n2t.net/addgene:17881 ; RRID:Addgene_17881) -
For your References section:
An essential switch in subunit composition of a chromatin remodeling complex during neural development. Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA, Crabtree GR. Neuron. 2007 Jul 19. 55(2):201-15. 10.1016/j.neuron.2007.06.019 PubMed 17640523
Map uploaded by the depositor.
