FLAG-mBAF45a IRES GFP
(Plasmid #17880)

• Depositing Lab: Jerry Crabtree
• Publication: Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Backbone

• Vector backbone: Beta-actin/CMV-IRES-EGFP
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAF45a
• Alt name: Phf10
• Species: M. musculus (mouse)
• Insert Size (bp): 1226
• Entrez Gene: Phf10 (a.k.a. 1810055P05Rik, AV024533)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • IRES EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: IRES-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry