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Addgene

PX459v3
(Plasmid #178799)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178799 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-Puro (PX459) V2.0 (Plasmid #62988)
  • Backbone manufacturer
    Dr. Feng Zhang's Lab
  • Backbone size (bp) 9174
  • Modifications to backbone
    gRNA scaffold sequence modified for increased gRNA expression (V3 scaffold)
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter Cbh (Cas9-2A Puro) and U6 (gRNA)
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.19.604224 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX459v3 was a gift from Paul Thomas (Addgene plasmid # 178799 ; http://n2t.net/addgene:178799 ; RRID:Addgene_178799)

  • For your References section:

    Enhancing gRNA Transcript levels by Reducing the Scaffold Poly-T Tract for Optimal SpCas9- and SaCas9-mediated Gene Editing. Chey YCJ, Gierus L, Lushington C, Arudkumar JC, Geiger A, Staker LG, Robertson LJ, Pfitzner C, Kennedy JG, Lee RHB, Godahewa GI, Thomas PQ, Adikusuma F. bioRxiv 2024.07.19.604224 10.1101/2024.07.19.604224
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