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Addgene

pJH4725
(Plasmid #178796)

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Item Catalog # Description Quantity Price (USD)
Plasmid 178796 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBSK
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 7439
  • Total vector size (bp) 11210
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DAF-2
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    3773
  • GenBank ID
  • Entrez Gene
    daf-2 (a.k.a. CELE_Y55D5A.5)
  • Promoter Pdpy-30

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer ATCCcccgggATGAATATTGTCAGATGTCG
  • 3′ sequencing primer ATggtaccACAAGTGGATGCTCATTATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH4725 was a gift from Mei Zhen (Addgene plasmid # 178796 ; http://n2t.net/addgene:178796 ; RRID:Addgene_178796)

  • For your References section:

    A Caenorhabditis elegans developmental decision requires insulin signaling-mediated neuron-intestine communication. Hung WL, Wang Y, Chitturi J, Zhen M. Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 2014 Mar 26. 10.1242/dev.103846 PubMed 24671950
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