pAAV-hGFAP-Pink Flamindo-WPRE-SV40
(Plasmid
#178790)
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PurposeAAV vector to express the red cAMP indicator in astrocytes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-hGFAP-*-WPRE-SV40
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePink Flamindo
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Insert Size (bp)1176
- Promoter hGFAP
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: The 5' ITR is missing in this plasmid, but the depositing lab was able to make functional AAV.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hGFAP-Pink Flamindo-WPRE-SV40 was a gift from Tetsuya Kitaguchi (Addgene plasmid # 178790 ; http://n2t.net/addgene:178790 ; RRID:Addgene_178790) -
For your References section:
Distinct temporal integration of noradrenaline signaling by astrocytic second messengers during vigilance. Oe Y, Wang X, Patriarchi T, Konno A, Ozawa K, Yahagi K, Hirai H, Tsuboi T, Kitaguchi T, Tian L, McHugh TJ, Hirase H. Nat Commun. 2020 Jan 24;11(1):471. doi: 10.1038/s41467-020-14378-x. 10.1038/s41467-020-14378-x PubMed 31980655