pBJ5 BRG1 DN
(Plasmid #17874)

• Depositing Lab: Jerry Crabtree
• Publication: Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Backbone

• Vector backbone: pBJ5
• Backbone size w/o insert (bp): 3300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brg DN
• Alt name: BRG1
• Alt name: BAF 190a
• Species: H. sapiens (human)
• Mutation: dominant negative
• Entrez Gene: SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pcDL-F

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dominant negative Brg, which we now know is a gain-of-function since it gives a phenotype that is more severe than the knock out of both Brg and Brm. Hence, we no longer recommend that the dominant negative be used to analyze the function of this complex.