pBJ5 hBRG1
(Plasmid #17873)

• Depositing Lab: Jerry Crabtree
• Publication: Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Backbone

• Vector backbone: pBJ5
• Backbone size w/o insert (bp): 3300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brg
• Alt name: BRG1
• Alt name: BAF 190a
• Species: H. sapiens (human)
• Mutation: R1608Q
• Entrez Gene: SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI/SalI (destroyed during cloning)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pcDL-F

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is an expression vector for the full length BRG/BAF190 protein which is similar to Drosphila Brm and contains the conserved ATPase domain in the yeast SWI2/SNF2 protein.

Addgene QC NGS analysis identifies an R1608Q mutation relative to NP_001122316.1.