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Addgene

pBJ5 mCnB
(Plasmid #17872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBJ5
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CnB
  • Alt name
    calcineurin B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    600
  • Entrez Gene
    Ppp3r1 (a.k.a. CaNB1, Cnb1, MCIP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pcDL-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBJ5 mCnB was a gift from Jerry Crabtree (Addgene plasmid # 17872 ; http://n2t.net/addgene:17872 ; RRID:Addgene_17872)

  • For your References section:

    Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25. 357(6380):695-7. 10.1038/357695a0 PubMed 1377362
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