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Addgene

A1-LCD-20G+20S+7F-7Y
(Plasmid #178688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    A1-LCD-20G+20S+7F-7Y
  • Species
    H. sapiens (human)
  • Mutation
    twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe
  • Entrez Gene
    HNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A1-LCD-20G+20S+7F-7Y was a gift from Tanja Mittag (Addgene plasmid # 178688 ; http://n2t.net/addgene:178688 ; RRID:Addgene_178688)

  • For your References section:

    Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains. Bremer A, Farag M, Borcherds WM, Peran I, Martin EW, Pappu RV, Mittag T. Nat Chem. 2022 Feb;14(2):196-207. doi: 10.1038/s41557-021-00840-w. Epub 2021 Dec 20. 10.1038/s41557-021-00840-w PubMed 34931046
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