A1-LCD+23G-23S
(Plasmid
#178685)
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PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST17
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameA1-LCD+23G-23S
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SpeciesH. sapiens (human)
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Mutationtwenty-three Ser residues replaced with Gly
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Entrez GeneHNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
A1-LCD+23G-23S was a gift from Tanja Mittag (Addgene plasmid # 178685 ; http://n2t.net/addgene:178685 ; RRID:Addgene_178685) -
For your References section:
Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains. Bremer A, Farag M, Borcherds WM, Peran I, Martin EW, Pappu RV, Mittag T. Nat Chem. 2022 Feb;14(2):196-207. doi: 10.1038/s41557-021-00840-w. Epub 2021 Dec 20. 10.1038/s41557-021-00840-w PubMed 34931046