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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFATc3
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Alt nameNF-ATc3
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SpeciesM. musculus (mouse)
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Insert Size (bp)3000
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Entrez GeneNfatc3 (a.k.a. C80703, D8Ertd281e, NFAT4, NFATx)
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Tag
/ Fusion Protein
- EE (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full-length mNFATc3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS mNFATc3-EE was a gift from Jerry Crabtree (Addgene plasmid # 17868 ; http://n2t.net/addgene:17868 ; RRID:Addgene_17868) -
For your References section:
Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature. Graef IA, Chen F, Chen L, Kuo A, Crabtree GR. Cell. 2001 Jun 29. 105(7):863-75. 10.1016/S0092-8674(01)00396-8 PubMed 11439183
Map uploaded by the depositor.