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Addgene

pCEP4 MR1 (H83P)
(Plasmid #178652)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10381
  • Total vector size (bp) 11470
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1089
  • Mutation
    H83P
  • GenBank ID
    NP_001522.1
  • Entrez Gene
    MR1 (a.k.a. HLALS)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Signal peptide from hemagglutinin (N terminal on insert)
    • Extracellular c-myc epitope ta (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4 MR1 (H83P) was a gift from Erik Procko (Addgene plasmid # 178652 ; http://n2t.net/addgene:178652 ; RRID:Addgene_178652)

  • For your References section:

    TAPBPR employs a ligand-independent docking mechanism to chaperone MR1 molecules. McShan AC, Devlin CA, Papadaki GF, Sun Y, Green AI, Morozov GI, Burslem GM, Procko E, Sgourakis NG. Nat Chem Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 10.1038/s41589-022-01049-9. 10.1038/s41589-022-01049-9 PubMed 35725941
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