Skip to main content
Addgene

pCEP4 TAPBPR-TM-TN6
(Plasmid #178650)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10381
  • Total vector size (bp) 11782
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAPBPR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1401
  • Mutation
    E205K, R207E, Q209S, Q272S, switched transmembrane domain with that of MHC-I
  • GenBank ID
    BC015017.2
  • Entrez Gene
    TAPBPL (a.k.a. TAPBP-R, TAPBPR)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • Signal/leader sequence from HLA class I histocompatibility antigen A-2 alpha chain (HLA) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4 TAPBPR-TM-TN6 was a gift from Erik Procko (Addgene plasmid # 178650 ; http://n2t.net/addgene:178650 ; RRID:Addgene_178650)

  • For your References section:

    TAPBPR employs a ligand-independent docking mechanism to chaperone MR1 molecules. McShan AC, Devlin CA, Papadaki GF, Sun Y, Green AI, Morozov GI, Burslem GM, Procko E, Sgourakis NG. Nat Chem Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 10.1038/s41589-022-01049-9. 10.1038/s41589-022-01049-9 PubMed 35725941
Related items: