pmcherry α-catenin ΔVBS
(Plasmid #178649)

• Purpose: express α-catenin without a vinculin binding site (VBS)
• Depositing Lab: Daniel Conway
• Publication: Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071. doi: 10.1091/mbc.E22-02-0071.

Backbone

• Vector backbone: pmcherryC1
• Backbone size w/o insert (bp): 4721
• Total vector size (bp): 7400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: α-catenin
• Alt name: CTNNA1
• Species: H. sapiens (human)
• Mutation: VBS of alpha catenin was removed and replaced with the equivalent domain of vinculin
• GenBank ID: NM_001903
• Entrez Gene: CTNNA1 (a.k.a. CAP102, MDBS2, MDPT2)
• Tag / Fusion Protein:
  • mcherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bglii (unknown if destroyed)
• 3′ cloning site: ecori (unknown if destroyed)
• 5′ sequencing primer: mcherryfwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry