FLIPrbs Q235A
(Plasmid
#17862)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepRSET-B
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameFLIPrbs Q235A
-
Alt nameFLIPrib-4u
-
Alt nameECFP-RBP-EYFP
-
Alt nameRibose Binding Protein
-
SpeciesE. coli
-
Insert Size (bp)2400
-
MutationQ235A
-
Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Q235A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FLIPrbs Q235A was a gift from Wolf Frommer (Addgene plasmid # 17862 ; http://n2t.net/addgene:17862 ; RRID:Addgene_17862) -
For your References section:
Development of a fluorescent nanosensor for ribose. Lager I, Fehr M, Frommer WB, Lalonde S. FEBS Lett. 2003 Oct 9. 553(1-2):85-9. 10.1016/S0014-5793(03)00976-1 PubMed 14550551