pEN-R2-mCsfGFP-L3
(Plasmid #178602)

• Purpose: It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.
• Depositing Lab: Frederica Theodoulou
• Publication: Zhang et al Plant Physiol. 2019 Jun;180(2):718-731. doi: 10.1104/pp.19.00051. Epub 2019 Mar 14.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Thermo fisher scientific
• Backbone size w/o insert (bp): 2500
• Total vector size (bp): 4100
• Vector type: Entry vector for gateway cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tandem fluorescent protein timers (tFTs)
• Alt name: mCherry-sfGFP timer
• Tag / Fusion Protein:
  • mCherry superfolder GFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 F
• 3′ sequencing primer: M13 R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: p415-GAL1-Ubi-R-mCherry-sfGFP (pMaM107; Khmelinskii and Knop, 2014)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Khmelinskii A, Knop M (2014) Analysis of protein dynamics with tandem
fluorescent protein timers. Methods Mol Biol 1174: 195–210