FLIPrbs
(Plasmid #17860)

• Depositing Lab: Wolf Frommer
• Publication: Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

Backbone

• Vector backbone: pRSET-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLIPrbs
• Alt name: FLIPrib-250n
• Alt name: ECFP-RBP-EYFP
• Alt name: Ribose Binding Protein
• Species: E. coli
• Insert Size (bp): 2400
• Mutation: D208G
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A RBP PCR product from genomic E. coli DNA encoding mature RBP was cloned into the KpnI site of FLIPglu-600u in pRSETB, replacing the glucose sensor's mglB moiety. The RBP sequence was found to carry a D208G substitution, which does not significantly affect the binding affinity.