hACE2
(Plasmid #1786)

• Purpose: Mammalian expression of human ACE2
• Depositing Lab: Hyeryun Choe
• Publication: Li et al Nature. 2003 Nov 27. 426(6965):450-4.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Angiotensin-converting enzyme 2
• Alt name: ACE2
• Alt name: SARS Receptor
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• GenBank ID: NM_021804
• Entrez Gene: ACE2 (a.k.a. ACEH)
• Tag / Fusion Protein:
  • C9 (GTETSQVAPA) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 40 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

See author's map for more information.

Information for Cloning Grade DNA (Catalog # 1786-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here