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Addgene

pWP001
(Plasmid #178596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAF256
  • Backbone manufacturer
    W. K. Smits, Leiden University Medical Center
  • Total vector size (bp) 8667
  • Modifications to backbone
    The Ptet promoter in pAP256 has been replaced with the C. difficile-derived xylose-inducible promoter and the hupA fragment has been replaced with a 292bp sequence encoding the cell-wall binding domain of endolysin CD16/50L
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CBD-SmBiT/LgBiT
  • Species
    C. difficile / synthetic
  • Promoter Pxyl

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CTGGACTTCATGAAAAACTAAAAAAAATATTG
  • 3′ sequencing primer CACCGACGAGCAAGGCAAGACCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please also cite:
Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(18)31160-4. doi: 10.1016/j.jmb.2019.01.001.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWP001 was a gift from Robert Fagan (Addgene plasmid # 178596 ; http://n2t.net/addgene:178596 ; RRID:Addgene_178596)

  • For your References section:

    Potential Role of the Host-Derived Cell-Wall Binding Domain of Endolysin CD16/50L as a Molecular Anchor in Preservation of Uninfected Clostridioides difficile for New Rounds of Phage Infection. Phothichaisri W, Chankhamhaengdecha S, Janvilisri T, Nuadthaisong J, Phetruen T, Fagan RP, Chanarat S. Microbiol Spectr. 2022 Apr 4:e0236121. doi: 10.1128/spectrum.02361-21. 10.1128/spectrum.02361-21 PubMed 35377223
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