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HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244
(Plasmid #17859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET_B
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Haemophilus influenzae heat shock loci U and V
  • Alt name
    H. influenzae HslUV
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
    1470
  • Mutation
    coding sequence for residues 108-243 of HslU (the "I domains") replaced with coding sequence for two glycines

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert for full-length HslUV cloned from two plasmids of the H. influenzae genomic sequencing project obtained from ATCC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also used to express and purify HslV protein alone, which is expressed in excess over HslU.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244 was a gift from David McKay (Addgene plasmid # 17859 ; http://n2t.net/addgene:17859 ; RRID:Addgene_17859)

  • For your References section:

    Structure and reactivity of an asymmetric complex between HslV and I-domain deleted HslU, a prokaryotic homolog of the eukaryotic proteasome. Kwon AR, Kessler BM, Overkleeft HS, McKay DB. J Mol Biol. 2003 Jul 4. 330(2):185-95. 10.1016/S0022-2836(03)00580-1 PubMed 12823960
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