pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2
(Plasmid
#178574)
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PurposeLentiviral vector for CamKIIα::Opto-vTrap
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178574 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti-CaMKIIa-ChETA-EYFP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCRY2PHR-P2A-CIBN-mCherry-VAMP2
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Alt nameOpto-vTrap
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Insert Size (bp)3174
- Promoter CaMKIIa
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2 was a gift from Won Do Heo (Addgene plasmid # 178574 ; http://n2t.net/addgene:178574 ; RRID:Addgene_178574) -
For your References section:
Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior. Won J, Pankratov Y, Jang MW, Kim S, Ju YH, Lee S, Lee SE, Kim A, Park S, Lee CJ, Heo WD. Neuron. 2022 Feb 2;110(3):423-435.e4. doi: 10.1016/j.neuron.2021.11.003. Epub 2021 Nov 30. 10.1016/j.neuron.2021.11.003 PubMed 34852235