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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR4-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFATc1
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Alt nameNF-ATc
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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Entrez GeneNFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
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Tag
/ Fusion Protein
- EE (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full-length huNFATc1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR4 hNFATc1-EE was a gift from Jerry Crabtree (Addgene plasmid # 17857 ; http://n2t.net/addgene:17857 ; RRID:Addgene_17857) -
For your References section:
NF-AT components define a family of transcription factors targeted in T-cell activation. Northrop JP, Ho SN, Chen L, Thomas DJ, Timmerman LA, Nolan GP, Admon A, Crabtree GR. Nature. 1994 Jun 9. 369(6480):497-502. 10.1038/369497a0 PubMed 8202141
Map uploaded by the depositor.