H2B-sfGFP-T2A-iCre
(Plasmid #178546)

• Purpose: pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT
• Depositing Lab: Mario Capecchi
• Publication: Additional gene targeting vector construction plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox ; FLP/FRT

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Histone H2B-sfGFP-T2A-iCre
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • * H2B-sfGFP-T2A-iCre-bpA-FNF.gb
• A portion of this plasmid was derived from a plasmid made by: CMV promoter is from Invitrogen. HIST1H2BB is from Addgene plasmid #20972. sfGFP is from Sandia Biotech. iCre is from Rolf Sprengel. bGH pA is from Neal Copeland. FRT-Neo*-FRT is from Addgene plasmid #22687.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The iCre in this plasmid has an extra Valine upstream of the SV40 nuclear localization signal that does not affect the function (similar to Addgene plasmids #113837 and #113849).

The inverted repeats of the bGH polyA sequences near the middle of the plasmid is prone to deletion by recombination. The depositor recommends checking specifically for this before using.