pBAD_TatABmCherryC
(Plasmid #178545)

• Purpose: pTatABC with an mCherry fluorescent protein tag at the end of TatB.
• Depositing Lab: Siegfried Musser
• Publication: Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2.

Backbone

• Vector backbone: pBAD22
• Backbone size w/o insert (bp): 4596
• Total vector size (bp): 6979
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TatA, TatBmCherry, TatC
• Species: Escherichia coli and Discoma sp.
• Insert Size (bp): 2383
• GenBank ID: NC_000913.3 AAV52164.1
• Promoter: araC
• Tag / Fusion Protein:
  • mCherry fused to TatB (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: TCTCTACTGTTTCTCCAT
• 3′ sequencing primer: GCGTTCTGATTTAATCTG

Resource Information

• Supplemental Documents:
  • pTatABcherryC.gb
• A portion of this plasmid was derived from a plasmid made by: Yahr & Wickner, 2001, EMBO J. 20:2472-2479.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construction of this vector is described in Whitaker N, Bageshwar UK, Musser SM.
Kinetics of precursor interactions with the bacterial Tat translocase detected by real-time FRET. J Biol Chem. 2012 Mar 30;287:11252-60; PubMed ID: 22315217; DOI:10.1074/jbc.M111.324525