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Addgene

pTatAkBxCs
(Plasmid #178544)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178544 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD22
  • Backbone size w/o insert (bp) 4596
  • Total vector size (bp) 6138
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TatA, TatB, TatC
  • Species
    Escherichia coli
  • Insert Size (bp)
    1586
  • GenBank ID
    NC_000913.3
  • Promoter araC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer TCTCTACTGTTTCTCCAT
  • 3′ sequencing primer CTGTATCAGGCTGAAAAT
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Yahr & Wickner, 2001, EMBO J. 20:2472-2479.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generated by modifying the pTatABC vector (Yahr & Wickner, 2001, EMBO J. 20:2472-2479) using the Quickchange Protocol. This plasmid contains a KpnI restriction site after TatA, an XbaI site after TatB, and a SalI site after TatC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTatAkBxCs was a gift from Siegfried Musser (Addgene plasmid # 178544 ; http://n2t.net/addgene:178544 ; RRID:Addgene_178544)

  • For your References section:

    Oligomerization state of the functional bacterial twin-arginine translocation (Tat) receptor complex. Sharma A, Chowdhury R, Musser SM. Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2. 10.1038/s42003-022-03952-2 PubMed 36123532
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