pET-28a mScarlet
(Plasmid #178543)

• Purpose: The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.
• Depositing Lab: Siegfried Musser
• Publication: Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5306
• Total vector size (bp): 6002
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mScarlet
• Species: Synthetic; construct
• GenBank ID: APD76535
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: taatacgactcactatagg
• 3′ sequencing primer: gtggtggtggtggtggtg

Resource Information

• Supplemental Documents:
  • pET28a_mScarlet.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene #85066

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mScarlet coding sequence was PCR amplified with an N-terminal 6xHis tag from pCytERM_mScarlet_N1 (Addgene #85066) and inserted into the vector fragment of pET28a (Novagen #69864) digested with NcoI and BamHI.