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Addgene

pCMMP-MCS-IRES-eGFP-P2A-iCas9
(Plasmid #178533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMMP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iCaspase9
  • Species
    H. sapiens (human)
  • Mutation
    FKBP12 F36 to V Truncated Caspase9 (135-416; lacking pro-domain)
  • Entrez Gene
    CASP9 (a.k.a. APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains F64L and V163A mutations in EGFP. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMMP-MCS-IRES-eGFP-P2A-iCas9 was a gift from Ryoji Yao (Addgene plasmid # 178533 ; http://n2t.net/addgene:178533 ; RRID:Addgene_178533)

  • For your References section:

    A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids. Okamoto T, Natsume Y, Yamanaka H, Fukuda M, Yao R. STAR Protoc. 2021 Sep 16;2(4):100780. doi: 10.1016/j.xpro.2021.100780. eCollection 2021 Dec 17. 10.1016/j.xpro.2021.100780 PubMed 34585151
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