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pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
(Plasmid #178528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB (piggyBac transposase donor vector)
  • Backbone size w/o insert (bp) 3450
  • Total vector size (bp) 9775
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rtTA, SspB-mScarlet-I-MYPT (1-169aa)
  • Alt name
    rtTA2S-M2-G72P-SE
  • Species
    H. sapiens (human), Synthetic
  • Promoter CAG, Tet responsive element

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence and plasmid map is available in the following benchling link (https://benchling.com/s/seq-xf8zdl8VLNsyQHRmCXqC)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169 was a gift from Kazuhiro Aoki (Addgene plasmid # 178528 ; http://n2t.net/addgene:178528 ; RRID:Addgene_178528)

  • For your References section:

    Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N, Sawai S, Kondo Y, Aoki K. Nat Commun. 2021 Dec 8;12(1):7145. doi: 10.1038/s41467-021-27458-3. 10.1038/s41467-021-27458-3 PubMed 34880255
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