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pCAGGS-PPP1CB-miRFP703
(Plasmid #178524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178524 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4720
  • Total vector size (bp) 6664
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP1c-miRFP703
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    PPP1CB (a.k.a. HEL-S-80p, MP, NSLH2, PP-1B, PP1B, PP1beta, PP1c, PPP1CD, PPP1beta)
  • Promoter CAG

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence and plasmid map is available in the following benchling link (https://benchling.com/s/seq-8370yYN7igj1pyuf3TeZ)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-PPP1CB-miRFP703 was a gift from Kazuhiro Aoki (Addgene plasmid # 178524 ; http://n2t.net/addgene:178524 ; RRID:Addgene_178524)

  • For your References section:

    Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N, Sawai S, Kondo Y, Aoki K. Nat Commun. 2021 Dec 8;12(1):7145. doi: 10.1038/s41467-021-27458-3. 10.1038/s41467-021-27458-3 PubMed 34880255
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