pTatABmNeonC
(Plasmid #178464)

• Purpose: pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.
• Depositing Lab: Siegfried Musser
• Publication: Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2.

Backbone

• Vector backbone: pBAD22
• Backbone size w/o insert (bp): 4596
• Total vector size (bp): 6892
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TatA, TatBmNeon, TatC
• Species: Escherichia coli and Branchiostoma lanceolatum
• Insert Size (bp): 2296
• GenBank ID: NC_000913.3 AGG56535.1
• Promoter: araC
• Tag / Fusion Protein:
  • mNeonGreen fused to TatB (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: TCTCTACTGTTTCTCCAT
• 3′ sequencing primer: CTGTATCAGGCTGAAAAT

Resource Information

• Supplemental Documents:
  • pTatABmNeonC.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mNeonGreen coding sequence was PCR amplified from pH6-mNeon (Addgene #178460) with KpnI and SpeI ends and inserted into the vector fragment of pTatABcherryC (Addgene #178545) digested with the same enzymes.