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Addgene

pTatABmNeonC
(Plasmid #178464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD22
  • Backbone size w/o insert (bp) 4596
  • Total vector size (bp) 6892
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TatA, TatBmNeon, TatC
  • Species
    Escherichia coli and Branchiostoma lanceolatum
  • Insert Size (bp)
    2296
  • GenBank ID
    NC_000913.3 AGG56535.1
  • Promoter araC
  • Tag / Fusion Protein
    • mNeonGreen fused to TatB (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer TCTCTACTGTTTCTCCAT
  • 3′ sequencing primer CTGTATCAGGCTGAAAAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mNeonGreen coding sequence was PCR amplified from pH6-mNeon (Addgene #178460) with KpnI and SpeI ends and inserted into the vector fragment of pTatABcherryC (Addgene #178545) digested with the same enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTatABmNeonC was a gift from Siegfried Musser (Addgene plasmid # 178464 ; http://n2t.net/addgene:178464 ; RRID:Addgene_178464)

  • For your References section:

    Oligomerization state of the functional bacterial twin-arginine translocation (Tat) receptor complex. Sharma A, Chowdhury R, Musser SM. Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2. 10.1038/s42003-022-03952-2 PubMed 36123532
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