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Addgene

pH6-mNeon
(Plasmid #178460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5303
  • Total vector size (bp) 6014
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Species
    Branchiostoma lanceolatum
  • Insert Size (bp)
    711
  • GenBank ID
    KC295282.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer ggccccaaggggttatgc
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene #69146

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mNeonGreen coding sequence was PCR amplified from L40C-CRISPR.EFS.mNeon (Addgene #69146) and inserted into the vector fragment of pET28a_mScarlet (Addgene #178543) digested with SpeI and BamHI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH6-mNeon was a gift from Siegfried Musser (Addgene plasmid # 178460 ; http://n2t.net/addgene:178460 ; RRID:Addgene_178460)

  • For your References section:

    Oligomerization state of the functional bacterial twin-arginine translocation (Tat) receptor complex. Sharma A, Chowdhury R, Musser SM. Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2. 10.1038/s42003-022-03952-2 PubMed 36123532
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