pcDNA-Mirk (pHX9F)
(Plasmid #17846)

• Depositing Lab: Eileen Friedman
• Publication: Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

Backbone

• Vector backbone: pcDNA3.1/HisA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mirk
• Alt name: Dyrk1B
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• GenBank ID: AAF15893
• Entrez Gene: DYRK1B (a.k.a. AOMS3, MIRK)
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • Anti-XPress (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert is an StuI fragment of the mirk/dyrk1b gene. The EcoRV site was destroyed during cloning, but a new BamHI site was generated at the 3' end.

This plasmid was named pHX9F in the original paper. "After EcoRI digestion, mirk cDNA was ligated into pcDNA3.1/HisA, yielding the hexahistidine fusion plasmid pHX9."