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pcDNA-Mirk (YF)
(Plasmid #17845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mirk
  • Alt name
    Dyrk1B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    Y271/273F
  • GenBank ID
    AAF15893
  • Entrez Gene
    DYRK1B (a.k.a. AOMS3, MIRK)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • Anti-XPress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert is an StuI fragment of the mirk/dyrk1b gene. The EcoRV site was destroyed during cloning, but a new BamHI site was generated at the 3' end.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Mirk (YF) was a gift from Eileen Friedman (Addgene plasmid # 17845 ; http://n2t.net/addgene:17845 ; RRID:Addgene_17845)

  • For your References section:

    Mirk protein kinase is a mitogen-activated protein kinase substrate that mediates survival of colon cancer cells. Lee K, Deng X, Friedman E. Cancer Res. 2000 Jul 1. 60(13):3631-7. PubMed 10910078
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